Ahmed T. Alahmar
University of Manchester, UK
Title: Skin expression of advanced glycation end products (AGEs), RAGE and Glyoxalase-I in patients with 40 years of diabetes
Biography
Biography: Ahmed T. Alahmar
Abstract
Objective:
To assess skin distribution and expression of AGEs, RAGE and GLO-I in patients with 40 years of diabetes mellitus and to correlate these expressions with measures of small and large nerve fibre damage.
Methodology:
Sixty-seven patients with type 1 diabetes mellitus of short (<15 years, n=20), intermediate (15-40 years, n=25) and long (>40 years, n=22) duration and 34 nondiabetic controls underwent diabetic neuropathy assessment: Neuropathy disability score (NDS), quantitative sensory testing (QST) including vibration pressure and thermal thresholds, nerve conduction studies (NCS), deep breathing heart rate variability (DB-HRV), corneal confocal microscopy (CCM) and intraepidermal nerve fibre density (IENFD) and AGEs, RAGE and GLO1-I expression in foot skin biopsies.
Results:
Patients with 40 years of diabetes demonstrated higher skin AGEs and RAGE and lower GLO-I expression. Patients with 40 years of diabetes who escaped diabetic neuropathy exhibited lower skin AGEs and RAGE and higher GLO-I expression in the epidermis, microvessels and basement membrane as compared to those with neuropathy and these expressions correlated with IENFD, CCM and NCS measures.
Conclusions:
Patients with 40 years of diabetes and no diabetic neuropathy have decreased skin AGEs and RAGE and increased GLO-I expression suggesting a potential role for these macromolecules as aetiological, marker of the disease as well as therapeutic target for diabetic neuropathy.